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Analysis of Amphiregulin (Human) by ELISA (CAT#: STEM-MB-0659-LGZ)

Introduction

Amphiregulin, AREG is an epidermal growth factor (EGF) -like molecule belonging to the EGF family. It is an autocrine growth factor and a mitogen of astrocytes, Schwann cells, and fibroblasts. AREG gene expression and release is caused by a variety of stimuli, including inflammatory lipids, cytokines, hormones, growth factors, and exogenous drugs. Physiologically, AREG plays an important role in lung morphogenesis and the development and maturation of mammary gland, bone tissue and oocyte. AREG also plays a key role in the restoration of tissue integrity after infection or injury.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Cell culture supernatant, Plasma
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