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Analysis of Amphiregulin (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0021-LGZ)

Introduction

Amphiregulin, AREG is an epidermal growth factor (EGF) -like molecule belonging to the EGF family. It is an autocrine growth factor and a mitogen of astrocytes, Schwann cells, and fibroblasts. AREG gene expression and release is caused by a variety of stimuli, including inflammatory lipids, cytokines, hormones, growth factors, and exogenous drugs. Physiologically, AREG plays an important role in lung morphogenesis and the development and maturation of mammary gland, bone tissue and oocyte. AREG also plays a key role in the restoration of tissue integrity after infection or injury.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma
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