Analysis of ANGPTL4 / PGAR / ARP4 / NL2 by ELISA (CAT#: STEM-MB-1548-LGZ)

Introduction

Angiopoietin-like proteins, also known as angiopoietin-associated proteins, are structurally related to angiopoietin. In humans, ANGPTL4 is a protein encoded by the AngpTL4 gene. The gene was also previously known as ANGPTL2, HFARP, PGAR or FIAF, but has been renamed ANGPTL4. ANGPTL4 is a member of the angiopoietin-like gene family and encodes secretory glycosylated proteins. ANGPTL4 is highly expressed in adipose tissue. During hypoxia, the expression is up-regulated in endothelial cells and cardiomyocytes. ANGPTL4 is involved in regulating fat and glucose metabolism, and it is also involved in blood vessel formation. ANGPTL4 is also expressed in hypoxic human tissues and in various cancers such as liposarcoma, hepatocellular carcinoma and conventional renal cell carcinoma.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer, Cardiovascular

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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