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Analysis of ApoE (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0034-LGZ)

Introduction

Apolipoprotein E (APOE), a secreted protein synthesized by liver cells and brain tissues, binds to a variety of receptors on the surface of different cells and plays a key role in lipid metabolism, immune regulation and neurology. ApoE is a polymorphic protein involved in the transformation and metabolism of lipoprotein, and its genes can regulate many biological functions, which is related to the pathogenesis of many eye diseases. The research on APOE and its gene polymorphism is one of the hot spots in medical research, and the exploration of the internal relationship between the two has important clinical application value for the prevention, diagnosis and treatment of eye diseases.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Metabolic

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma
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