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Analysis of Apolipoprotein M/Apo M by ELISA (CAT#: STEM-MB-1564-LGZ)

Introduction

Apolipoprotein M (Apo M) is encoded by the APOM gene in humans. The protein encoded by the gene is an apolipoprotein, a member of the Apolipoprotein family. Apo M was found to be associated with high density lipoproteins, and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. Apo M is secreted through the plasma membrane, but still binds to the membrane and participates in lipid transport. It was found that the APOM gene has two transcribed variants encoding two different isomers, but only one of them has been fully identified. The average molecular weight of Apo M was 21253 Da and that of the single isotope was 21239 Da.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Diabetes Mellitus, Metabonomics

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples
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