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Analysis of Layilin/LAYN by ELISA (CAT#: STEM-MB-1579-LGZ)

Introduction

Layilin (LAYN), a 55 kDa transmembrane protein homologous to C-type lectins, is present in many cell lines and tissue extracts. LAYN specifically binds hyaluronic acid, but not the other mucopolysaccharides tested, through its extracellular domain (a component of the extracellular matrix ubiquitously present in most animal tissues and body fluids). Studies have shown that LAYN may mediate signals from the extracellular matrix to the cytoskeleton through interactions with different intracellular binding elements, thereby participating in the modulation of cell cortical structure.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

LAYN plays a role in many cellular processes, including cell morphology, adhesion, motility, homeostasis, and signal transduction. In addition, LAYN may play an important role in the invasion and lymphatic metastasis of lung cancer.

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples
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