Analysis of AQP12B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1552-LGZ)

Introduction

Official Full Name: aquaporin 12B<br />Also known as: INSSA3<br />Predicted to enable channel activity. Predicted to be involved in transmembrane transport. Predicted to be integral component of membrane. Predicted to be active in cytoplasm.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Red Sea Bream Iridovirus (RSIV) by Real-Time PCR Method (CAT#: STEM-MB-3467-LGZ)
Analysis of Staphylococcus Aureus by Real-Time PCR Method (CAT#: STEM-MB-3369-LGZ)
Analysis of SNAP47 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0314-LGZ)
Analysis of DIPK1B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2021-LGZ)
Analysis of NIBAN1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0487-LGZ)
Analysis of TMOD4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0724-LGZ)
Analysis of Tropiladaps Clareae by Real-Time PCR Method (CAT#: STEM-MB-3743-LGZ)
Analysis of Chlamydophila Pneumoniae by Real-Time PCR Method (CAT#: STEM-MB-2663-LGZ)