Analysis of TAF1A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0299-LGZ)

Introduction

Official Full Name: TATA-box binding protein associated factor, RNA polymerase I subunit A<br />Also known as: SL1; RAFI48; TAFI48; MGC:17061<br />This gene encodes a subunit of the RNA polymerase I complex, Selectivity Factor I (SLI). The encoded protein is a TATA box-binding protein-associated factor that plays a role in the assembly of the RNA polymerase I preinitiation complex. Alternate splicing results in multiple transcript variants encoding multiple isoforms.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of BLM Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2477-LGZ)
Analysis of ENO1-AS1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1317-LGZ)
Analysis of Arillus Longan by Real-Time PCR Method (CAT#: STEM-MB-3958-LGZ)
Analysis of Moraxella Bovoculi by Real-Time PCR Method (CAT#: STEM-MB-3812-LGZ)
Analysis of PSMB4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0611-LGZ)
Analysis of RBBP5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0349-LGZ)
Analysis of OR2T6 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0540-LGZ)
Analysis of OR2G2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0529-LGZ)