Analysis of PFKFB2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0550-LGZ)

Introduction

Official Full Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
Also known as: PFK-2/FBPase-2
The protein encoded by this gene is involved in the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule controlling glycolysis in eukaryotes. The encoded protein has 6-phosphofructose-2 kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and fructose-2,6-bisphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while related enzymes encoded by different genes regulate fructose-2,6-bisphosphate levels in the liver and muscles. This enzyme acts as a homodimer. Two transcript variants of this gene have been found encoding two different isoforms.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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