Analysis of RAB11FIP1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1890-LGZ)

Introduction

Official Full Name: RAB11 family interacting protein 1<br />Also known as: RCP; NOEL1A; rab11-FIP1<br />This gene encodes one of the rab11 family interacting proteins (Rab11-FIPs), which plays a role in rab11-mediated vesicle recycling. The encoded protein may be involved in endophageal sorting, trafficking of proteins including integrin subunits and epidermal growth factor receptor (EGFR), and trafficking between circulating endosomes and the trans-Golgi network. Alternative splicing results in multiple transcript variants. Pseudogenes are described on the X chromosome.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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