Analysis of NSMCE2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1885-LGZ)

Name: Analysis of NSMCE2 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1885-LGZ
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Introduction

Official Full Name: NSE2 (MMS21) homolog, SMC5-SMC6 complex SUMO ligase
Also known as: NSE2; MMS21; ZMIZ7; C8orf36
This gene encodes a member of the E3 small ubiquitin-associated modifier (SUMO) ligase family that mediates the attachment of SUMO proteins to proteins involved in nuclear transport, transcription, chromosome segregation, and DNA repair. The encoded protein is part of the chromosome structure maintenance (SMC) 5/6 complex, which plays a key role in genome maintenance, promoting chromosome segregation and inhibiting mitotic recombination. Knockout of the mouse homolog before embryonic day 10.5 is lethal. Naturally occurring mutations in this gene, which disrupt SUMO ligase activity, are associated with primitive dwarfism and extreme insulin resistance.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.