Inquiry Basket

Analysis of SETX Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1945-LGZ)

Introduction

Official Full Name: senataxin
Also known as: ALS4; AOA2; STEX; Sen1; SCAN2; SCAR1; bA479K20.2
This gene encodes a protein named for its homology to the Sen1p protein of fungi which has RNA helicase activity encoded by a domain at the C-terminal end of the protein. The protein encoded by this gene contains a DNA/RNA helicase domain at its C-terminal end which suggests that it may be involved in both DNA and RNA processing. Mutations in this gene have been associated with ataxia-ocular apraxia-2 (AOA2) and an autosomal dominant form of juvenile amyotrophic lateral sclerosis (ALS4).

Add to Cart Please Inquire

Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Gyrodactylus Salaris by Real-Time PCR Method (CAT#: STEM-MB-3852-LGZ)
Analysis of CEBPZ Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1800-LGZ)
Analysis of Salmonella Arizonae by Real-Time PCR Method (CAT#: STEM-MB-3401-LGZ)
Analysis of APCS Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0253-LGZ)
Analysis of Brevibacillus Laterosporus by Real-Time PCR Method (CAT#: STEM-MB-2738-LGZ)
Analysis of Whitewater Arroyo Virus (WWAV) by Real-Time PCR Method (CAT#: STEM-MB-3641-LGZ)
Analysis of CPLANE2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1302-LGZ)
Analysis of Ovine Adenovirus by Real-Time PCR Method (CAT#: STEM-MB-3026-LGZ)

We use cookies to understand how you use our site and to improve the overall user experience. This includes personalizing content and advertising. Read our Privacy Policy

Accept Cookies