Analysis of Salmonella Arizonae by Real-Time PCR Method (CAT#: STEM-MB-3401-LGZ)

Introduction

Salmonellae are Gram-negative, non-spore-forming, facultatively anaerobic bacilli belonging to the family of Enterobacteriaceae, which usually cause food-bornediseases. Salmonella arizonae, one of the less commonmembers of Salmonellae, has the distinguishing biochem-ical characteristics of the ability to ferment lactose, utilizemalonate, liquefy gelatin, and inhibition by the presence ofpotassium cyanide.
A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.