Analysis of H2AC25 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1023-LGZ)

Introduction

Official Full Name: H2A clustered histone 25
Also known as: H2AW; HIST3H2A
Histones are fundamental nuclear proteins responsible for the structure of eukaryotic chromosome fibers in the nucleosome. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). By linking DNA interactions between histone H1 and nucleosomes, chromatin fibers are further compacted to form higher-order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the H2A histone family. The transcript of this gene contains a palindromic termination element.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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