Analysis of Streptococcus Oralis by Real-Time PCR Method (CAT#: STEM-MB-3135-LGZ)

Introduction

Streptococcus oralis was the original name given to isolates from the human oral cavity. Currently, the name S. oralis is used to indicate a well-defined species belonging to the S. mitis group which includes Streptococcus australis, Streptococcus cristatus, Streptococcus gordonii, Streptococcus infantis, Streptococcus lactarius, Streptococcus massiliensis, S. mitis, Streptococcus oligofermentas, S. oralis, Streptococcus parasanguinis, Streptococcus peroris, S. pneumonia, S. sanguinis, and Streptococcus sinensis, which are mainly isolated from the normal oral biota in man.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.