Analysis of HBB-3'HS1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2243-LGZ)

Introduction

Gene description: beta-globin 3' hypersensitive site 1
Gene type: biological region
Also known as: HSVI; 3'HS1
This element represents a DNase I hypersensitivity site found approximately 20 kb downstream of the erythrocyte β-globin gene cluster. It has been shown to function as a scaffold attachment region, and it also has enhancer-blocking activity mediated by binding CTCF transcription factors. Chromosome conformation capture assays indicate that it is an integral part of the beta-globin active chromatin hub in erythroid cells, where it undergoes looping interactions with the beta-globin locus control region (LCR) and active beta-globin gene promoters. Mutations in this hypersensitive site may be associated with beta-thalassemia. This hypersensitive locus is located in a region that is deleted in certain beta-thalassemias, including several hereditary fetal hemoglobin (HPFH) deletions.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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