Analysis of HES6 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1581-LGZ)

Introduction

Official Full Name: hes family bHLH transcription factor 6<br />Also known as: HES-6; bHLHb41; bHLHc23; C-HAIRY1<br />This gene encodes a member of a subfamily of basic helix-loop-helix transcription repressors that have homology to the Drosophila enhancer of split genes. Members of this gene family regulate cell differentiation in numerous cell types. The protein encoded by this gene functions as a cofactor, interacting with other transcription factors through a tetrapeptide domain in its C-terminus. Alternatively spliced transcript variants encoding different isoforms have been described.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of GON4L Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1010-LGZ)
Analysis of Bacteroides Spp. by Real-Time PCR Method (CAT#: STEM-MB-2805-LGZ)
Analysis of REEP1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1676-LGZ)
Analysis of Cortex Magnoliae Officinalis by Real-Time PCR Method (CAT#: STEM-MB-3959-LGZ)
Analysis of Treponema Hyodysenteriae by Real-Time PCR Method (CAT#: STEM-MB-3198-LGZ)
Analysis of JMJD1C Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2090-LGZ)
Analysis of OPTC Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0383-LGZ)
Analysis of Human Immunodeficiency Virus 1 (HIV-1M-K) by Real-Time PCR Method (CAT#: STEM-MB-3222-LGZ)