Analysis of LRIF1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1201-LGZ)

Introduction

Official Full Name: ligand dependent nuclear receptor interacting factor 1<br />Also known as: RIF1; FSHD3; HBiX1; C1orf103<br />Predicted to enable retinoic acid receptor binding activity. Involved in dosage compensation by inactivation of X chromosome. Located in Barr body; centriolar satellite; and nucleoplasm. Colocalizes with chromosome, telomeric region.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of EPS8L3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1187-LGZ)
Analysis of SAG Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1783-LGZ)
Analysis of Adenovirus (AV) - Type D by Real-Time PCR Method (CAT#: STEM-MB-3448-LGZ)
Analysis of Human Rhinovirus 29 by Real-Time PCR Method (CAT#: STEM-MB-3276-LGZ)
Analysis of Ibaraki Virus by Real-Time PCR Method (CAT#: STEM-MB-3229-LGZ)
Analysis of PIGC Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0575-LGZ)
Analysis of C11orf58 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2406-LGZ)
Analysis of Lentinus edodes-Derived Material by Real-Time PCR Method (CAT#: STEM-MB-2876-LGZ)