Analysis of DVL1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1320-LGZ)

Introduction

Official Full Name: dishevelled segment polarity protein 1<br />Also known as: DVL; DRS2; DVL1L1; DVL1P1<br />DVL1, the human homolog of the Drosophila disheveled gene (dsh), encodes a cytoplasmic phosphorylated protein that regulates cell proliferation and serves as a sensor molecule for developmental processes, including cell division and neuroblast specification. DVL1 is a candidate gene for neuroblastoma transformation. Schwartz-Jampel syndrome and Charcott-Marie-tooth disease type 2A have been mapped to the same region as DVL1. The phenotypes of these disorders may be consistent with defects caused by aberrant expression of DVL genes during development.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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