Analysis of Tai Forest Ebola Virus (TAFV) by Real-Time PCR Method (CAT#: STEM-MB-3313-LGZ)

Introduction

Taï Forest ebolavirus (CIEBOV / TAFV)belongs to genus Ebola virus which has other four species including Zaire Ebolavirus (ZEBOV / EBOV); Reston ebolavirus (REBOV / RESTV) and Bundibugyo ebolavirus (BEBOV / BDBV), Sudan eoblavirus (SUDV / SEBOV).Taï Forest ebolavirus is one of four ebolaviruses that causes Ebola virus Disease / Ebola hemorrhagic Fever (EVD / EHF) in humans.
A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.

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Principle Applications Procedure Materials Notes Related Products

Principle

Primers and other components are optimized with high sensitivity. We designed positive controls to distinguish false negative samples. PCR is highly specific, and primers are designed according to highly conserved areas, which do not cross-react with other DNA/RNA.

Applications

It can be used for both qualitative and quantitative detection. The linear range is at least 5 orders of magnitude when used for quantitative detection.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

• Sample Type: DNA

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