Analysis of COQ8A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0884-LGZ)

Introduction

Official Full Name: coenzyme Q8A
Also known as: COQ8; ADCK3; ARCA2; CABC1; SCAR9; COQ10D4
This gene encodes a mitochondrial protein similar to yeast ABC1, which functions in an electron-transferring membrane protein complex in the respiratory chain. It is not related to the family of ABC transporter proteins. Expression of this gene is induced by the tumor suppressor p53 and in response to DNA damage, and inhibiting its expression partially suppresses p53-induced apoptosis. Alternatively spliced transcript variants have been found; however, their full-length nature has not been determined.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of GABRD Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1334-LGZ)
Analysis of LCE1E Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1119-LGZ)
Analysis of CSGALNACT1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1920-LGZ)
Analysis of Pasteurella Multocida by Real-Time PCR Method (CAT#: STEM-MB-3632-LGZ)
Analysis of SDHC Gene (Mutation) in Gastrointestinal Stromal Tumor by RT-qPCR (CAT#: STEM-MT-0196-LGZ)
Analysis of Mycoplasma Mycoides Subsp (Mycoides SC) by Real-Time PCR Method (CAT#: STEM-MB-3020-LGZ)
Analysis of Porcine Torque Teno Virus (PTTV) by Real-Time PCR Method (CAT#: STEM-MB-3946-LGZ)
Analysis of SNORD14B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2431-LGZ)