Analysis of SLC39A4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1860-LGZ)

Introduction

Official Full Name: solute carrier family 39 member 4
Also known as: AEZ; ZIP4; AWMS2
This gene encodes a member of the zinc/iron-regulated transporter-like protein (ZIP) family. The encoded protein localizes to cell membranes and is required for zinc uptake in the intestine. Mutations in this gene result in acrodermatitis enteropathica. Multiple transcript variants encoding different isoforms have been found for this gene.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of LCLAT1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1817-LGZ)
Analysis of CLIC3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2023-LGZ)
Analysis of F11R Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0946-LGZ)
Analysis of IFI16 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1035-LGZ)
Analysis of Treponema Pallidum Subsp. Pallidum by Real-Time PCR Method (CAT#: STEM-MB-3187-LGZ)
Analysis of PLCH2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1367-LGZ)
Analysis of ANKRD65 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1261-LGZ)
Analysis of Raillietina Tetragona by Real-Time PCR Method (CAT#: STEM-MB-4051-LGZ)