Analysis of Vibrio Alginolyticus by Real-Time PCR Method (CAT#: STEM-MB-3061-LGZ)

Introduction

Vibrio alginolyticus is a halophilic organism formerly regarded as biotype 2 of V. parahaemolyticus. It fails to grow on CLED agar but grows in the presence of 10% sodium chloride. It forms large, yellow (sucrose-fermenting) colonies on TCBS. There is pronounced swarming on non-selective solid media.
A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.