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Analysis of DDHD1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2497-LGZ)

Introduction

Official Full Name: DDHD domain containing 1<br />Also known as: SPG28; PAPLA1; iPLA1I; PA-PLA1; iPLA1alpha<br />This gene is a member of the intracellular phospholipase A1 gene family. The protein encoded by this gene preferentially hydrolyzes phosphatidic acid. It is a cytoplasmic protein with a mitochondrial localization and is thought to be involved in the regulation of mitochondrial dynamics. Overexpression of this gene results in disruption of mitochondrial tubule structure, whereas deletion of this gene results in elongation of mitochondrial tubules. In male mice, loss of this gene caused reproductive defects due to disruption of mitochondrial organization during sperm formation. In humans, mutations in this gene are associated with hereditary spastic paraplegia (HSP), also known as Strumpell-Lorrain disease, or familial spastic paraplegia (FSP). This genetic disorder is characterized by progressive weakness and leg cramps. Alternative splicing results in multiple transcript variants encoding different isoforms.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements