Analysis of GNB1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1333-LGZ)

Introduction

Official Full Name: G protein subunit beta 1
Also known as: MDS; HG2A; MRD42
Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternative splicing results in multiple transcript variants.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of S100A5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0331-LGZ)
Analysis of LZTS1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1915-LGZ)
Analysis of OR10R2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0505-LGZ)
Analysis of CAMKMT Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1718-LGZ)
Analysis of Didymella lycopersici Klebahn by Real-Time PCR Method (CAT#: STEM-MB-2579-LGZ)
Analysis of Sin Nombre Virus (SNV) by Real-Time PCR Method (CAT#: STEM-MB-3580-LGZ)
Analysis of Corynebacterium Minutissimum by Real-Time PCR Method (CAT#: STEM-MB-2621-LGZ)
Analysis of KCNK12 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1137-LGZ)