Analysis of MIR34A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1339-LGZ)

Introduction

Official Full Name: microRNA 34a
Also known as: mir-34; MIRN34A; mir-34a; miRNA34A
microRNAs (miRNAs) are short (20-24nt) non-coding RNAs that participate in the post-transcriptional regulation of gene expression in multicellular organisms by affecting mRNA stability and translation. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs), which can be protein-coding or non-coding. The primary transcript is cleaved by Drosha RNase III to generate an approximately 70 nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by cytoplasmic Dicer RNase to generate mature miRNA and antisense miRNA star (miRNA*) products. Mature miRNAs are incorporated into the RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base-pairing with miRNAs, most commonly resulting in translational repression or destabilization of target mRNAs. RefSeq represents predicted microRNA stem-loops. This miRNA is a member of the highly conserved miR-34 family. This miRNA acts as a tumor suppressor, and dysregulation or loss of host genes that process this miRNA is associated with cancer progression in many cell types.