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Analysis of IL10 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1071-LGZ)

Introduction

Official Full Name: interleukin 10<br />Also known as: CSIF; TGIF; GVHDS; IL-10; IL10A<br />The protein encoded by this gene is a cytokine that is mainly produced by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immune regulation and inflammatory responses. Down-regulate the expression of Th1 cytokines, MHC class II Ags and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-κB activity and participate in the regulation of JAK-STAT signaling pathway. Knockout studies in mice revealed that this cytokine is an essential immune modulator in the gut. Mutations in this gene have been associated with increased susceptibility to HIV-1 infection and rheumatoid arthritis.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements