Analysis of TRIM6 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2179-LGZ)

Introduction

Official Full Name: tripartite motif containing 6<br />Also known as: RNF89<br />The protein encoded by this gene is a member of the TRIM family. The TRIM motif includes three zinc-binding domains, RING, B-box type 1 and B-box type 2 domains, and a coil region. The protein localizes to the nucleus, but its specific function has not been determined. The gene was mapped to chromosome 11p15, in the TRIM gene cluster. Alternative splicing results in multiple transcript variants. Readable transcripts from this gene to the downstream TRIM34 gene were also observed, leading to fusion products of these neighboring family members.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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