Analysis of MLLT11 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0460-LGZ)

Introduction

Official Full Name: MLLT11 transcription factor 7 cofactor
Also known as: AF1Q
Different sign genes for ALL1, HRX or MLL located on 11q23 have been shown to fuse with many translocation partners in leukemia cases. In two children with acute myeloid leukemia, the t(1;11)(q21;q23) translocation was shown to fuse the MLL gene to a gene on chromosome band 1q21. The N-terminal portion of the MLL gene is critical for leukemogenesis in a translocation involving band 11q23. This gene encodes 90 amino acids. It is highly expressed in thymus but not in peripheral lymphoid tissue. In contrast to its limited distribution in normal hematopoietic tissues, the gene is expressed in all leukemic cell lines.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of C1orf74 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0821-LGZ)
Rapid EGFR Mutation Analysis of Fine Needle Aspirates from Non-Small-Cell Lung Cancer by RT-qPCR (CAT#: STEM-MT-0016-LGZ)
Analysis of Nocardia Otitidiscaviarum by Real-Time PCR Method (CAT#: STEM-MB-3305-LGZ)
Analysis of PLPBP Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1887-LGZ)
Analysis of GPR89B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1030-LGZ)
Analysis of NUF2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0508-LGZ)
Arbitrary PCR (AP-PCR) / RAPD (CAT#: STEM-MB-0223-WXH)
Analysis of DENND2C Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1147-LGZ)