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Analysis of ARF1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0256-LGZ)

Introduction

Official Full Name: ADP ribosylation factor 1<br />Also known as: PVNH8<br />ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. Members of this family encode small guanine nucleotide-binding proteins that stimulate cholera toxin ADP-ribosyltransferase activity and function as activators of phospholipase D in vesicle trafficking. The gene product includes 6 ARF proteins and 11 ARF-like proteins, constituting a family of the RAS superfamily. ARF proteins are classified into class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), with members of each class sharing a common genetic organization. The ARF1 protein localizes to the Golgi apparatus and plays a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants of this gene encoding the same protein have been found.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements