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Analysis of ARID1A Gene (Mutation) in Hepatocellular Carcinoma by RT-qPCR (CAT#: STEM-MT-0093-LGZ)

Introduction

Also known as: ELD; B120; CSS2; OSA1; P270; hELD; BM029; MRD14; hOSA1; BAF250; C1orf4; BAF250a; SMARCF1<br />This gene encodes a member of the SWI/SNF family that has helicase and ATPase activities and is thought to regulate the transcription of certain genes by altering the chromatin structure surrounding these genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for the transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that may be important for its function. First, it has a DNA-binding domain that specifically binds AT-rich DNA sequences known to be recognized by the SNF/SWI complex at the β-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. The protein encoded by this gene is thought to confer specificity to the SNF/SWI complex and may recruit the complex to its targets through protein-DNA or protein-protein interactions. Two transcript variants of this gene have been found to encode different isoforms.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Hepatocellular carcinoma

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements