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Analysis of ATF6 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0782-LGZ)

Introduction

Official Full Name: activating transcription factor 6<br />Also known as: ACHM7; ATF6A<br />This gene encodes a transcription factor that activates target genes of the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein embedded in the endoplasmic reticulum. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor in quiescent rather than proliferating squamous carcinoma cells. There are conflicting reports on the association of polymorphisms of this gene with diabetes in different populations, but another polymorphism is associated with elevated plasma cholesterol levels. This gene is also considered a potential therapeutic target for cystic fibrosis.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements