Analysis of B4GALNT1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2478-LGZ)

Introduction

Official Full Name: beta-1,4-N-acetyl-galactosaminyltransferase 1
Also known as: GALGT; SPG26; GALNACT; GalNAc-T
GM2 and GD2 gangliosides are sialic acid-containing glycosphingolipids. GalNAc-T is the enzyme involved in the biosynthesis of G(M2) and G(D2) glycosphingolipids. GalNAc-T catalyzes the transfer of GalNAc into G(M3) and G(D3) by a beta-1,4 linkage, resulting in the synthesis of G(M2) and G(D2), respectively. Three transcript variants encoding different isoforms have been found for this gene.

Add to Cart Please Inquire

Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of ABCB10 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0749-LGZ)
Analysis of PEA15 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0555-LGZ)
Analysis of Camel-Derived Material by Real-Time PCR Method (CAT#: STEM-MB-2756-LGZ)
Analysis of Trichophyton Rubrum by Real-Time PCR Method (CAT#: STEM-MB-3338-LGZ)
Analysis of Rabies Virus (RV、street virus) by Real-Time PCR Method (CAT#: STEM-MB-3107-LGZ)
Analysis of Melissococcus Plutonius by Real-Time PCR Method (CAT#: STEM-MB-3567-LGZ)
Analysis of PUSL1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1396-LGZ)
Analysis of Amycolaptosis Spp. by Real-Time PCR Method (CAT#: STEM-MB-3277-LGZ)