Analysis of BIRC6 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1795-LGZ)

Introduction

Official Full Name: baculoviral IAP repeat containing 6<br />Also known as: BRUCE; APOLLON<br />This gene encodes a protein with a BIR (baculoviral inhibition of apoptosis protein repeat) domain and a UBCc (ubiquitin-conjugating enzyme E2, catalytic) domain. This protein inhibits apoptosis by facilitating the degradation of apoptotic proteins by ubiquitination.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Human Herpesvirus-6B (HHV-6B) by Real-Time PCR Method (CAT#: STEM-MB-3329-LGZ)
Analysis of Canine Adenovirus (CAV) by Real-Time PCR Method (CAT#: STEM-MB-2700-LGZ)
Analysis of MAL2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1903-LGZ)
Analysis of CAMKMT Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1718-LGZ)
Analysis of Human Immunodeficiency Virus 1 (HIV-1M-H) by Real-Time PCR Method (CAT#: STEM-MB-3223-LGZ)
Analysis of Bovine Adenovirus (BAV-3) by Real-Time PCR Method (CAT#: STEM-MB-3225-LGZ)
Analysis of OR52A1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2347-LGZ)
Analysis of CNIH3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0860-LGZ)