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Analysis of LAMTOR2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1096-LGZ)

Introduction

Official Full Name: late endosomal/lysosomal adaptor, MAPK and MTOR activator 2<br />Also known as: p14; ENDAP; ROBLD3; HSPC003; MAPBPIP; MAPKSP1AP; Ragulator2<br />The product of this gene is highly conserved with a mouse protein associated with the cytoplasmic face of late endosomes and lysosomes. The mouse protein interacts with MAPK scaffolding protein 1, a component of the mitogen-activated protein kinase pathway In humans, mutations in this gene are associated with primary immunodeficiency syndrome and suggest a role for this protein in endosomal biogenesis. Multiple transcript variants of this gene have been found encoding different isoforms.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements