Analysis of C1orf56 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0239-LGZ)

Introduction

Also known as: MENT
This gene is a proto-oncogene whose promoter is methylated by DNA methyltransferase 3B (DNMT3B), which represses the proto-oncogene. However, a catalytically inactive isoform of DNMT3B is overexpressed in lymphomas, leading to hypomethylation of the proto-oncogene's promoter and derepression of the proto-oncogene.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of SLC2A5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1401-LGZ)
Analysis of Squama Manis by Real-Time PCR Method (CAT#: STEM-MB-3945-LGZ)
Analysis of Caprine Herpesvirus (CpHV) by Real-Time PCR Method (CAT#: STEM-MB-2680-LGZ)
Analysis of Treponema Vincentii by Real-Time PCR Method (CAT#: STEM-MB-3194-LGZ)
Analysis of HBB-HS-111 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2241-LGZ)
Analysis of ARL8A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0255-LGZ)
Analysis of Staphylococcus Hominis by Real-Time PCR Method (CAT#: STEM-MB-3377-LGZ)
Analysis of FIRRM Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0974-LGZ)