Analysis of BMP-4 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0061-LGZ)

Introduction

Recombinant Human Bone Morphogenetic Protein 4 (BMP-4) is a bone morphogenic protein that constitutes a subfamily of the TGF-β superfamily of structurally related signaling proteins. BMP-4 is also involved in the development and maintenance of bone and cartilage. Reduced expression of BMP-4 is associated with many skeletal disorders, including hereditary dysplastic ossification disorders.

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Principle Applications Procedure Materials Notes Related Products

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Bone Disorders

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma

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