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Analysis of BMP-9/GDF-2 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0065-LGZ)

Introduction

Bone morphogenetic protein (BMP), also known as bone morphogenic protein, is a group of highly conserved functional proteins with similar structures, belonging to the TGF-β family. BMP can stimulate DNA synthesis and cell replication, thereby promoting the directional differentiation of mesenchymal cells into osteoblasts. BMP9 is also known as growth differentiation factor (GDF)-2. BMP9 is mainly produced by hepatic stellate cells and participates in the regulation of various biological functions, including iron ion balance, cartilage formation, angiogenesis, neuronal differentiation, glucose and lipid metabolism, etc.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Bone Disorders

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma
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