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Analysis of BMPR2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1508-LGZ)

Introduction

Official Full Name: bone morphogenetic protein receptor type 2
Also known as: BMR2; PPH1; BMPR3; BRK-3; POVD1; T-ALK; BMPR-II
This gene encodes a member of the bone morphogenetic protein (BMP) receptor transmembrane serine/threonine kinase family. The ligands for this receptor are members of the TGF-β superfamily. BMP is involved in endochondral bone formation and embryogenesis. These proteins signal by forming a heterogeneous complex of two different types of serine (threonine) kinase receptors: type I receptors of approximately 50-55 kd and type II receptors of approximately 70-80 kd. Mutations in this gene have been associated with primary pulmonary hypertension (familial and fenfluramine-associated) and pulmonary veno-occlusive disease.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements
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