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Analysis of MUTYH Gene (Mutation) in Hereditary Cancer-Predisposing Syndrome by RT-qPCR (CAT#: STEM-MT-0102-LGZ)

Introduction

Also known as: MYH<br />This gene encodes a DNA glycosylase involved in DNA oxidative damage repair. At sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine (a major oxidative damage to DNA damage). This enzyme excises the adenine base from the DNA backbone. This protein is localized to the nucleus and mitochondria. This gene product is thought to function as an apoptotic signal in the introduction of single-strand breaks following oxidative damage. Mutations in this gene lead to a genetic predisposition to colorectal cancer known as mutyh-associated polyposis (MAP). Multiple transcript variants encoding different isoforms have been found for this gene.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Hereditary cancer-predisposing syndrome

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements