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Analysis of C4BPB Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0818-LGZ)

Introduction

Official Full Name: complement component 4 binding protein beta<br />Also known as: C4BP<br />This gene encodes a member of a protein superfamily consisting mainly of short consensus repeats arranged in tandem of approximately 60 amino acids. A unique beta chain encoded by this gene assembles with seven identical alpha chains to form the major isoform of C4b-binding protein, a multimeric protein that controls the activation of the complement cascade through the classical pathway. The C4B-binding protein also has a regulatory role in the coagulation system, mediated through the binding of the beta chain of protein S, a vitamin K-dependent protein, that acts as a cofactor for the activation of protein C. On human chromosome 1, the genes encoding the α and β chains are adjacent to each other in the regulation of the complement activation gene cluster. Alternative splicing produces multiple transcript variants.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements