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Analysis of CACNA1E Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0816-LGZ)

Introduction

Official Full Name: calcium voltage-gated channel subunit alpha1 E
Also known as: BII; CACH6; DEE69; gm139; Cav2.3; EIEE69; CACNL1A6
Voltage-dependent calcium channels are multisubunit complexes composed of α-1, α-2, β, and δ subunits in a 1:1:1:1 ratio. These channels mediate the entry of calcium ions into excitable cells and are involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division, and cell death. This gene encodes the α-1e subunit of the R-type calcium channel, which belongs to the "high voltage activated" group and may be involved in the regulation of neuronal firing patterns important for information processing. Alternative splice transcript variants encoding different isoforms have been described for this gene.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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