Analysis of CD84 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0852-LGZ)

Introduction

Official Full Name: CD84 molecule
Also known as: LY9B; hCD84; mCD84; SLAMF5
This gene encodes a membrane glycoprotein that is a member of the signaling lymphocyte activation molecule (SLAM) family. This family forms a subset of the larger CD2 cell-surface receptor Ig superfamily. The encoded protein is a homophilic adhesion molecule that is expressed in numerous immune cells types and is involved in regulating receptor-mediated signaling in those cells. Alternate splicing results in multiple transcript variants.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of PEX19 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0552-LGZ)
Analysis of PAQR6 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0533-LGZ)
Analysis of Mycobacterium Pinnipedii by Real-Time PCR Method (CAT#: STEM-MB-2959-LGZ)
Analysis of CTNNBIP1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1299-LGZ)
Analysis of RD3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0332-LGZ)
Analysis of KCNC4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1203-LGZ)
Analysis of NES Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0490-LGZ)
Analysis of RAB17-DT Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1646-LGZ)