Analysis of Cell Mechanics in Single Vinculin-Deficient Cells Using a Magnetic Tweezer (CAT#: STEM-MB-1242-WXH)

Introduction

Vinculin has no enzymatic activity. It regulates adhesion by directly binding to actin, stimulating actin polymerization and recruiting actin remodeling proteins. In the absence of vinculin, cell–matrix and cell–cell adhesion are dramatically impaired, indicating vinculin plays a critical role in human physiology.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Magnetic tweezers (MT) are scientific instruments for the manipulation and characterization of biomolecules or polymers. These apparatus exert forces and torques to individual molecules or groups of molecules. It can be used to measure the tensile strength or the force generated by molecules.

Applications

Study of mechanical properties of biological macromolecules like DNA or proteins in single-molecule experiments.
Study of the rheology of soft matter.
Study of force-regulated processes in living cells.

Procedure

1.Sample preparation
2.Force Calibration
3.Measurement
4.Analysis

Materials

Magnetic tweezers

Other recommended products

Eukaryotic membrane tethers by Magnetic tweezers (MT) (CAT#: STEM-MB-1252-WXH)
Measurement of Stickiness of extracellular polymeric substances by Magnetic tweezers (MT) (CAT#: STEM-MB-1249-WXH)
Study of RNAP II transcription initiation by Magnetic tweezers (MT) (CAT#: STEM-MB-1264-WXH)
Phagosomal Transport and Maturation by Magnetic tweezers (MT) (CAT#: STEM-MB-1275-WXH)
Study of the effect of Trimethylamine N-oxide (TMAO) on protein by Magnetic tweezers (MT) (CAT#: STEM-MB-1276-WXH)
Study of the Viscoelastic Properties of Fibrin Clots by Magnetic tweezers (MT) (CAT#: STEM-MB-1272-WXH)
Measurement of torsional stiffness in RecA-DNA filaments by Magnetic tweezers (MT) (CAT#: STEM-MB-1277-WXH)
DNA micromanipulation by Magnetic tweezers (MT) (CAT#: STEM-MB-1238-WXH)