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Analysis of CR1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0882-LGZ)

Introduction

Official Full Name: complement C3b/C4b receptor 1
Also known as: KN; C3BR; C4BR; CD35
This gene is a member of the complement-activating receptor (RCA) family and is located in the "cluster RCA" region of chromosome 1. The genome is polymorphic at this locus, with allele-specific splice variants encoding distinct alloforms based on the presence/absence of long homologous repeats (lhr). The gene encodes a single-generation type I membrane glycoprotein that is present in erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is an antigenic system located on this protein. This protein mediates the binding of cells to complement-activating particles and immune complexes. Reduced expression of this protein and/or mutations in this gene have been associated with gallbladder cancer, angiocapillary glomerulonephritis, systemic lupus erythematosus, sarcoidosis, and Alzheimer's disease. Mutations in this gene are also associated with reduced nodulation by P. falciparum, which protects people from severe malaria.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements
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