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Analysis of CTSS Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0901-LGZ)

Introduction

Official Full Name: cathepsin S<br />The preproprotein encoded by this gene is a member of the peptidase C1 family, a lysosomal cysteine protease involved in the degradation of antigenic proteins into polypeptides and presentation on MHC class II molecules. The mature protein cleaves the invariant chain of the MHC class II molecule in the endolysosomal compartment, allowing antigen-MHC class II complex formation and allowing MHC-II to properly display extracellular antigenic peptides. The mature protein also functions as elastase over a wide pH range. When secreted from cells, this protein remodels components of the extracellular matrix such as elastin, collagen, and fibronectin. This gene has been implicated in the pathology of many inflammatory and autoimmune diseases and plays an important role in some lung diseases due to its elastase activity. Alternative splice transcript variants of this gene have been found to encode distinct isoforms.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements