Analysis of Omsk Hemorrhagic Fever Virus (OHFV) by Real-Time PCR Method (CAT#: STEM-MB-2949-LGZ)

Introduction

Omsk hemorrhagic fever virus (OHFV) is a lesser-studied flavivirus found in central Russia. There are only about 100 to 200 cases annually in the endemic area. This virus is naturally harbored in small rodents and is spread to humans via Dermacentor spp. tick bites or from contact with rodent blood or excrement.<br />A real-time polymerase chain reaction (real-time PCR,or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time),not at its end,as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter,which permits detection only after hybridization of the probe with its complementary sequence.