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Analysis of CXCL16/SRPSOX by ELISA (CAT#: STEM-MB-1568-LGZ)

Introduction

Chemokine (C-X-C motif) ligand 16(CXCL16) is a small molecular weight cytokine belonging to the CXC chemokine family. It is composed of 254 amino acids and is a type I membrane protein. The human CXCL16 gene is located on chromosome 17. CXCL16 is produced by dendritic cells in the T-cell region of the lymphoid organs and cells in the red pulp of the spleen. The inflammatory cytokines IFN-γ and TNF-α can induce the expression of CXCL16. CXCL16 interacts with the chemokine receptor CXCR6, also known as Bonzo. Injection of folic acid forced CXCL 16 to methylate, inducing high levels of methylation by the CXCL 16 gene promoter in the colon, ileum, and lungs, while also causing iNKT cells to accumulate in these tissues.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples
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