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Analysis of IgG1 (Mouse) by ELISA (CAT#: STEM-MB-0922-LGZ)

Introduction

Immunoglobulin G (IgG) is a type of antibody produced and released by plasma B cells. IgG is the main type of antibody in blood and extracellular fluid, which can control the infection of human tissue. Antibodies protect the body from infection by binding to many pathogens, such as viruses, bacteria and fungi. In humans, IgG has four subtypes IgG1, IgG2, IgG3 and IgG4. In mice and rats, IgG also has four subtypes—IgG1, IgG2a, IgG2b, and IgG3.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Assay Development

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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