Unlock Exclusive Discounts & Flash Sales! Click Here to Join the Deals on Every Wednesday!

Analysis of AChE (Human) by ELISA (CAT#: STEM-MB-0646-LGZ)

Introduction

Acetylcholinesterase (AchE) is a key enzyme in biological nerve conduction. In the cholinergic synapses, ACHE can degrade acetylcholine, stop the excitatory effect of neurotransmitters on the postsynaptic membrane, and ensure the normal transmission of nerve signals in the biological body. It has the activity of carboxypeptidase and aminopeptidase. Acetylcholinesterase is involved in cell development and maturation, and can promote neuronal development and nerve regeneration.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Neurobiology/Neurodegeneration, Toxicology

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids
Advertisement